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Electrochemical Oxidation of Flavonoids at a Nano- Metal Oxide Modified Carbon Paste Electrodes and Determination Their Levels in Human Urine

[ Vol. 9 , Issue. 1 ]


Lai-Hao Wang and Hsiu-Hua Liu   Pages 143 - 149 ( 7 )


We constructed nano-metal oxide (tin oxide, cerium oxide, cobalt oxide, iron oxide, nickel oxide, gold oxide, and silver oxide) -modified carbon paste electrodes (CPEs) for detecting flavonols (kaempferol, myricetin, and quercetin) and flavones (luteolin and apigenin) using high-performance liquid chromatography with an electrochemical detector (HPLC-ECD). Flavonoids are more efficiently catalytically oxidized on a gold oxid e-modified CPE than on a bare CPE. For direct current mode, with the current at a constant potential, and measurements with suitable experimental parameters, a linear concentration from 40 to 1600 ng mL-1 was found. The limits of quantification for flavonoids were below 0.5 ng. The developed method detected flavonoids in urine samples. Findings using HPLC-ECD and HPLC with an ultraviolet detector were comparable.


Nano- metal oxide, Modified carbon paste electrodes, Flow-through voltammetric detector, Flavonoids, Human urine


Department of Medical Chemistry, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Jen Te, Tainan 71743, Taiwan.

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