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Detection of Phenolic Compounds by Tyrosinase Modified Clark Type Electrode

[ Vol. 11 , Issue. 1 ]

Author(s):

Margarita Stoytcheva, Roumen Zlatev, Mary Triny Beleno and Gisela Montero   Pages 50 - 55 ( 6 )

Abstract:


In this work is suggested a Clark type electrode with disposable enzyme membrane for phenols determination. This approach allows avoiding the electrode fouling by recording the oxygen reduction current, and obtaining a stable and reproducible sensor response. An appropriate method for enzyme membrane fabrication by cellulose acetate filters coating with tyrosinase through the use of biocompatible hydrogels was proposed and tested. The sensitivity of the catechol determination, as an example, was found to be 0.89 µA mmol-1 L. The detection limit equaled 1 µmol L-1. The achieved RSD was 1.22%.

Keywords:

Clark type electrode, tyrosinase, catechol, disposable membrane, hydrogel.

Affiliation:

Universidad Autónoma de Baja California, Instituto de Ingenieria, 21280 Mexicali, Mexico.

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