Submit Manuscript  

Article Details


A Derivative Method with Free Radical Oxidation to Predict Resveratrol Metabolites by Tandem Mass Spectrometry

[ Vol. 11 , Issue. 4 ]

Author(s):

Wangta Liu, Yow-Ling Shiue, Yi-Reng Lin, Hugo You-Hsien Lin and Shih-Shin Liang   Pages 300 - 306 ( 7 )

Abstract:


In this study, we demonstrated an oxidative method with free radical to generate 3,5,4 -trihydroxy-transstilbene (trans-resveratrol) metabolites and detect sequentially by an autosampler coupling with liquid chromatography electrospray ionization tandem mass spectrometer (LC-ESI–MS/MS). In this oxidative method, the free radical initiator, ammonium persulfate (APS), was placed in a sample bottle containing resveratrol to produce oxidative derivatives, and the reaction progress was tracked by autosampler sequencing. Resveratrol, a natural product with purported cancer preventative qualities, produces metabolites including dihydroresveratrol, 3,4 -dihydroxy-trans-stilbene, lunularin, resveratrol monosulfate, and dihydroresveratrol monosulfate by free radical oxidation. Using APS free radical, the concentrations of resveratrol derivatives differ as a function of time. Besides simple, convenient and time- and labor saving, the advantages of free radical oxidative method of its in situ generation of oxidative derivatives followed by LC-ESI–MS/MS can be utilized to evaluate different metabolites in various conditions.

Keywords:

Free radical, metabolite, resveratrol, ammonium persulfate (APS), liquid chromatography tandem mass spectrometry (LC–MS/MS), multiple reaction monitoring (MRM).

Affiliation:

Department of Biotechnology, Kaohsiung Medical University, P.O. Box: 80708 Kaohsiung, Taiwan.



Read Full-Text article