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Influence of Some Variables on Separation of Diastereomeric FVDA-Amino Alcohol Derivatives in High Performance Thin Layer Chromatography and Pressurized Planar Electrochromatography Systems

[ Vol. 12 , Issue. 4 ]

Author(s):

B. Polak, A. Chomicki, P. Wierzchowski and A. Klimek-Turek   Pages 335 - 348 ( 14 )

Abstract:


Separation of five -blocker 1-fluoro-2,4-dinitrophenyl-5-L-valine amide (FVDA) derivatives i.e. acebutolol, atenolol, metoprolol, oxprenolol and pindolol by means of high-performance thin layer chromatography (HPTLC) as well as pressurized planar electrochromatography (PPEC) techniques and with the use of HPTLC RP-18W plates as well as the acetonitrile - buffer mobile phase is presented.

The influence of concentration of acetonitrile and pH of the mobile phase buffer, and temperature on migration distance of the solute zones was investigated. It has been observed that an increase of acetonitrile content of the mobile phase affects the solute zone migrations and retentions in HPTLC and PPEC. Whereas rise of eluent buffer pH enhances predominantly the isomer migration distances in PPEC. The temperature increase results the enhancement of solute migration distances in PPEC and diminishes isomer retention in HPTLC. Moreover, it improves the diastereomer pair zones resolution in both investigated systems. What is more, the separation selectivity in PPEC and HPTLC system differs. The former system presents higher efficiency in comparison with the latter.

In addition separation of FVDA-oxprenolol and FVDA-pindolol isomers is presented for the first time.

Keywords:

Diastereomers, amino alcohols, separation, variables, HPTLC, PPEC.

Affiliation:

Department of Physical Chemistry, Medical University of Lublin, Chod ki 4A, 20-093 Lublin, Poland.



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