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Compared Chemical Activity and Effects on Cell Viability of Various Antioxidant Compounds

[ Vol. 13 , Issue. 2 ]

Author(s):

Céline Clayeux, Eric Marchioni, Remmelt Van der Werf, Stéphanie Dal, Séverine Sigrist, Damien Steyer and Saïd Ennahar*   Pages 104 - 110 ( 7 )

Abstract:


Background: Oxidative stress plays a major role in the development of several diseases. A healthy food diet, supplying the organism with antioxidants, is generally believed to help prevent this stress.

Methods: With the aim of screening several antioxidants commonly found in food, a method using β pancreatic cells and based on the reduction of a tetrazolium sodium salt into colored formazan in the presence of antioxidant compounds was used. In parallel, the same compounds were investigated using a chromatographic online antioxidant detection system (Coads) based on the detection of the reduced form of the radical cation 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) which is first allowed to react online with the eluting compounds.

Results: The obtained data suggested that most phenolic compounds possessed both antioxidant and prooxidant properties. Gallic acid, in particular, showed among the highest antioxidant activities with the Coads method (322±46 μM Trolox equivalent), but caused a decrease in cell viability (-25%).

Conclusion: Moreover, when screening natural compounds for their antioxidant activity, combining both chemical and biological methods is a more targeted approach compared with the single use of either method.

Keywords:

Oxidative stress, bioactive compounds, antioxidant, pro-oxidant, β pancreatic cells, ABTS.

Affiliation:

TWISTAROMA, 28 rue de Herrlisheim, 68021 Colmar, Equipe de Chimie Analytique des Molécules BioActives, UMR 7178 – IPHC, Université de Strasbourg - Faculté de Pharmacie 74, route du Rhin, 67400 Illkirch, Equipe de Chimie Analytique des Molécules BioActives, UMR 7178 – IPHC, Université de Strasbourg - Faculté de Pharmacie 74, route du Rhin, 67400 Illkirch, Centre européen d'étude du Diabète (CeeD), Hôpital de Hautepierre, Boulevard René Leriche 67200 Strasbourg, Centre européen d'étude du Diabète (CeeD), Hôpital de Hautepierre, Boulevard René Leriche 67200 Strasbourg, TWISTAROMA, 28 rue de Herrlisheim, 68021 Colmar, Equipe de Chimie Analytique des Molécules BioActives, UMR 7178 – IPHC, Université de Strasbourg - Faculté de Pharmacie 74, route du Rhin, 67400 Illkirch

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