Submit Manuscript  

Article Details


Optimization of Analytical Strategy for Determination of Total Antimony in Fish Muscle Tissue using Hydride Generation Atomic Absorption Spectrometry

[ Vol. 13 , Issue. 4 ]

Author(s):

Mariio M. Silva Junior, Danilo J. Leao, Laiana O.B. Silva, Catilea Franca Pimentel, Karina Santos Garcia and Sergio L.C. Ferreira   Pages 285 - 290 ( 6 )

Abstract:


Background: Antimony is a metaloide that offers human health risks. Then, the determination of this chemical element in food samples is always opportune. This paper proposes a method for determination of total antimony in fish muscle tissue using hydride generation atomic absorption spectrometry (HG AAS).

Methods: The optimization of the quantification step was performed using a two-level full factorial design and a Box Behnken matrix, involving the factors: nitric acid concentration, carrier gas flow (argon) and sodium borohydride concentration. The digestion procedure of the samples was established employing microwave assisted radiation using nitric acid.

Results: The method using the optimized conditions allows the determination of total antimony by the external calibration technique with limits of detection and quantification of 0.8 and 2.6 ng g-1 for a sample mass of 0.20 g, and precision expressed as relative standard deviation of 4.32%, for an antimony solution of concentration 5 μg L-1. The accuracy was confirmed by analysis of a certified reference material of oyster tissue furnished by National Institute of Standards and Technology (NIST).

Conclusion: The method was applied for determination of total antimony in seven samples of fish muscle tissue (Arraia - Brazilian name, Elasmobranquiomorpha rajiformes) acquired in two regions of the Todos os Santos Bay, Bahia, Brazil. The antimony content found varied from 62 to 370 ng g-1. These results are agreeing with other data reported by literature.

Keywords:

Antimony, fish muscle, HG AAS, experimental design, todos os santos bay, atomic absorption spectrometry, organic species.

Affiliation:

Instituto de Química, Universidade Federal da Bahia, Salvador, Bahia, Instituto de Química, Universidade Federal da Bahia, Salvador, Bahia, INCT de Energia e Ambiente, Universidade Federal da Bahia, Salvador, Bahia, Instituto de Geociências, Universidade Federal da Bahia, Salvador, Bahia, Instituto de Geociências, Universidade Federal da Bahia, Salvador, Bahia, Instituto de Química, Universidade Federal da Bahia, Salvador, Bahia

Graphical Abstract:



Read Full-Text article