Guzin Alpdogan*, Sule Dinc Zor and Nevim San Pages 291 - 298 ( 8 )
Background: This study describes a simple and rapid analytical method for the simultaneous determination of trace amounts of copper(II), zinc(II), nickel(II), cobalt(II), iron(II), cadmium(II) and mercury(II) in fish samples by the reversed-phase highperformance liquid chromatography (RP-HPLC) with di-2-pyridyl ketone benzoylhydrazone (DPKBH) as pre-column derivatization reagent.Methods: Seven metal chelates were successfully separated with methanol:acetonitrile:tetrabutyl ammonium bromide (1.0 mM):water mixture containing ammonium acetate buffer (pH 6.0) and DPKBH (1.0 mM) (40:24:1:30:5, v/v/v/v/v) as mobile phase on a C18 column at a flow rate 1.0 mL min-1 at 370 nm. Results: Under the optimum conditions, reproducibilities of analyte retention times (relative standard deviation (RSD) ≤ 0.04%) and peak areas (RSD ≤ 1.5%) were found to be good with the lineraties R2 ≥ 0.9995. The validation of the method was performed by the analysis of certified reference materials. Conclusion: The method developed was applied for the determination of Cu(II), Zn(II), Ni(II), Co(II), Fe(II), Cd(II) and Hg(II) in fish samples.
Chelating reagent, di-2-pyridyl ketone benzoylhydrazone, trace element, RP-HPLC, fish samples, derivatization reagent.
Yildiz Technical University, Department of Chemistry, Davutpasa Campus, 34220, Istanbul, Yildiz Technical University, Department of Chemistry, Davutpasa Campus, 34220, Istanbul, Yildiz Technical University, Department of Chemistry, Davutpasa Campus, 34220, Istanbul