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Liquid Chromatographic Determination of Malondialdehyde in Plasma Samples After Liquid–Liquid Microextraction

[ Vol. 14 , Issue. 4 ]

Author(s):

Sajad Azizi, Jafar Soleymani, Maryam Khoubnasabjafari, Azam Samadi and Abolghasem Jouyban*   Pages 416 - 422 ( 7 )

Abstract:


Background: Malondialdehyde is a product of lipid peroxidation of polyunsaturated fatty acids in foods and biological samples and widely used as biomarker of oxidative stress in various diseases. We describe the validation of a new microextraction-LC-UV method for the determination of malondialdehyde (MDA) in the form of 2,4-dinitrophenylhydrazine (DNPH) derivatization in plasma samples.

Objective: A new microextraction technique called vortex and N2 assisted liquid-liquid microextraction has been developed for the determination MDA in plasma samples.

Method: The DNPH-derivatized MDA was extracted into ethyl acetate as an extraction solvent and measured by LC-UV at 310 nm after evaporation of ethyl acetate and re-dissolving in mobile phase composed of 0.2% acetic acid–acetonitrile (50:50; v/v). This method validated according to U.S. Food and Drug Administration guidelines.

Results: The limit of detection of MDA was 0.038 µmol L-1 (2.74 µg L-1) and the intra and interday relative standard deviations were in the range of 3.8-5.0% and 5.5-9.4%, respectively for different concentrations of MDA.

Conclusion: A precise and valid method to measure MDA as DNPH derivatization in plasma samples using LC was proposed.

Keywords:

Malondialdehyde, 2, 4-dinitrophenylhydrazine, vortex assisted liquid liquid microextraction, N2 assisted liquid liquid microextraction, validation, samples.

Affiliation:

Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz

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