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Enantioseparation and Determination of Norepinephrine, Epinephrine and Isoproterenol by Capillary Electrophoresis-indirect Electrochemiluminescence in Human Serum

[ Vol. 14 , Issue. 5 ]


Yuefang Hu and Xiaoping Wei*   Pages 504 - 511 ( 8 )


Background: Two enantiomers of a drug might have various pharmacological, physiological, and toxicological properties was well known. Enantioseparation of chiral drugs is becoming significant in various fields.

Objective: To get better detection sensitivity and good enantioresolution of norepinephrine(NE), epinephrine (EP) and isoproterenol (ISO)at the same time.

Method: A new and sensitive method for NE, EP and ISO was developed by capillary electrophoresis-indirect electrochemilumine-scence (CE-IECL) based on their quenching effects on the Ru(bpy)32+/tripropylamine (Ru(bpy)32+/TPA) system, using negatively charged carboxymethyl-beta-cyclodextrin(CM-β-CD) as chiral selectors.

Results: The enantiomers of NE, EP and ISO were baseline enantioseparation and highly sensitive detection.

Conclusion: A wide and excellent linear relationship of each enantiomer in the range of 0.2 to 1000 µM with detection limit (S/N=3) from 0.06 to 0.1µM, the RSD of peak area and peak height from 3.1-3.5% and 2.9-3.4%, respectively. This CE-IECL method was successfully applied for the enantioseparation of NE, EP and ISO in serum sample, the recoveries of NE, EP and ISO in serum were ranged from 96.0 to 104% with relative standard deviations less than 3.3%.


Capillary electrophoresis-indirect electrochemiluminescence, Enantioseparation, norepinephrine, epinephrine, isoproterenol, chiral drugs.


College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China; College of Materials and Environmental engineering, Hezhou University, Hezhou, 542899, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004

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