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Magnetic Solid-Phase Extraction for the Determination of Ochratoxin A in Wine and Beer by HPLC-FLD

[ Vol. 14 , Issue. 2 ]

Author(s):

Peng Li, Fei Pei, Qin Liu and Yong Fang*   Pages 129 - 134 ( 6 )

Abstract:


Background: Reports on the mycotoxin ochratoxin A occurrence in wine and beer indicate that this is a worldwide problem due to the widespread consumption of these beverages.

Objective: The existing solid phase extraction methods for the ochratoxin A are usually used in the form of microcolumns, which are generally driven by pumps and take a long time to finish sample pretreatment. It is highly desirable to develop fast, reliable, and low-cost analytical procedures for the monitoring of ochratoxin A in wine and beer.

Method: A method comprising magnetic solid-phase extraction (MSPE) followed by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed for the quantification of ochratoxin A in wine and beer. Hexadecyl-immobilized silica-coated magnetic Fe3O4 (C16- Fe3O4@SiO2) nanoparticles were prepared by single-step sol-gel modification of bare Fe3O4 nanoparticles and were used as MSPE adsorbents.

Results: Key parameters that affect the MSPE of ochratoxin A in wine and beer, namely, the pH, content of organic components, and desorption solution, were investigated in detail. Under the optimized extraction conditions with gradient chromatographic separation, a limit of detection of 0.02 µg L-1, a precision of 4.4%, and recoveries in the range of 89.7–103.6% were achieved for the determination of ochratoxin A in wine and beer.

Conclusion: A MSPE method using C16-Fe3O4@SiO2 nanoparticles as the adsorbent in combination with HPLC-FLD was developed for the determination of ochratoxin A in wine and beer. The validated method is able to provide reliable date at the lower level, displaying satisfactory linearity, precision, accuracy and moderate cost.

Keywords:

Ochratoxin A, magnetic solid-phase extraction, HPLC-FLD, wine, beer, organic components.

Affiliation:

College of Food Science and Engineering, Nanjing University of Finance and Economics, Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing 210023, College of Food Science and Engineering, Nanjing University of Finance and Economics, Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing 210023, College of Food Science and Engineering, Nanjing University of Finance and Economics, Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing 210023, College of Food Science and Engineering, Nanjing University of Finance and Economics, Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing 210023

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