Shengzhong Rong, Deng Pan, Xuehui Li, Mucong Gao, Hongwei Yu, Jinghui Jiang, Ze Zhang, Dongdong Zeng, Hongzhi Pan* and Dong Chang* Pages 648 - 655 ( 8 )
Background: 8-Hydroxy-2’-deoxyguanosine (8-OHdG) has been regarded as a typical stable biomarker of DNA oxidative damage, and its level is one of the criteria for early diagnosis of various diseases. Considering the significance of 8-OhdG, various analytical techniques have been used for assaying 8-OHdG but all of them suffer from basic limitations like highly expensive instrumentation, large amount of sample requirement, complicated sample pre-treatment, tedious and time-consuming procedures etc. However, electroanalytical sensors provide a faster, easy and sensitive means of analyzing.
Methods: The chitosan (CS) film provided the high electrode activity and stability which is required for detecting 8-OHdG though direct electrochemical oxidation. Zirconia was employed because it has some unique properties, such as high redox activity and selectivity etc. High-performance composite was easily detected by differential pulse voltammetry at a working voltage of 0. 51 V (vs. Ag/AgCl). A rapid and sensitive electrochemical sensor based on CS and metal oxide nanocrystalline for the determination of 8-OHdG was established.
Results: Under optimized experimental conditions, the peak currents of differential pulse voltammetry increased as the concentrations of 8-OHdG increased from 10 to 200 ng·mL-1. The detection limit was 3.67 ng·mL-1 which was calculated by the S/N ratio of 3. The recoveries of the real spiked samples are in the range between 93.2 to 105.3%.
Conclusion: The electrochemical sensor for direct 8-OHdG determination using a new CS/zirconia composite for GCE modification was developed and showed excellent reproducibility, stability and sensitivity for the specific determination of 8-OHdG in real urine specimen.
Electrochemical sensor, Oxidative DNA damage, 8-Hydroxy-2’-desoxyguanosine, metal oxide nanocrystalline, CS, determination, electrochemical oxidation.
Public Health School, Mudanjiang Medical College, Mudanjiang, Heilongjiang, 157011, Medical School, Southeast University, Nanjing 210009, College of Public Administration, Nanjing Agricultural University Nanjing 210095, Public health school, Harbin Medical University, Harbin, Heilongjiang, 150081, Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399, Clinical Laboratory, the First Affiliated Hospital of Harbin Medical University, Harbin, 150001, Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399, Cooperation Study Center, School of Medicine and Technology, Shanghai University of Medical & Health Sciences, Shanghai, 201318, Cooperation Study Center, School of Medicine and Technology, Shanghai University of Medical & Health Sciences, Shanghai, 201318, Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399