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A Comparative Study of Determination the Spectral Characteristics of Serum Total Protein Among Laser System and Spectrophotometric: Advantage and Limitation of Suggested Methods

[ Vol. 15 , Issue. 5 ]


AL-Timimi Zahra*   Pages 583 - 590 ( 8 )


Background and Objective: Laser spectroscopy is becoming an increasingly paramount analytical tool. Scientists today have at their disposal many various types of laser-based analytical techniques. In this article, the possibility of using capabilities of a laser to analyze and find the concentration of Serum Total Protein (STP) was studied.

Materials and Methods: The laser system includes a diode laser with 532 nm wavelength, with maximum output power being 5 mW. Laser bandwidth ranges around (524 nm – 546 nm) experimentally justified using a monochromator. A simple variable resistance with a range from zero to10Ω for obtaining a range of laser output power, detector, parallel variable resistance with the range from zero to 5 kΩ and meter for measuring the percentage of transmittance. The absorption spectroscopy of STP samples was measured by double beam spectrophotometer.

Results: Maximum absorbance of STP is at the range (520-580 nm) and the peak at (500) nm. Laser system measurements included the study of absorbance of STP as a function of cuvet thickness, transmittance as a function of cuvet thickness and absorbance as a function of laser power. In order to ascertain our calculations, the results have been compared with the results of the spectrophotometer. The Relative Standard Deviation (RSD%) values are about (0.67-17.18).

Conclusion: The diode laser system is a highly efficient and easy system and allows access to a range of powers. Since the divergence of the laser beam is very low. All results are in good agreement with conventional double beam spectrophotometer.


Absorption spectroscopy, blood, laser spectroscopy, serum total protein, spectrophotometer, transmittance.


Laser Physics College of Science for Women, Babylon University, Hillah

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