Farzaneh Farazmand and Mahnaz Qomi* Pages 1 - 8 ( 8 )
Introduction: Verapamil (Verap) is an antidysrhythmic agent and a calcium channel blocker, indicated for angina, hypertension, supraventricular arrhythmias, and migraine. Objective: Drug monitoring plays a critical role in patient survival. In order to prevent the onset of drug toxicity, trace levels of this drug should be determined. Method: For this reason, solvent bar microextraction technique coupled with high-performance liquid chromatography was implemented. Results: Under optimum condition, verapamil was micro-extracted from a donor solution (pH=11) to an acceptor solution (pH=3.2). It was transferred through n-octanol as the organic solvent, which was impregnated in the pores of the hollow fiber. Salt addition (30%) had the major effect on the efficiency of the method. Interaction of time (65 min), temperature (25°C), and stirring rate (818 rpm) had a significant effect too. It all resulted in a limit of detection and quantification of 15 ng mL-1 and 50 ng mL-1, respectively. The relative standard deviations of analysis were 4.9% within a day (n=3) and 5.7% between days (n=9). The calibration curves represented good linearity for urine and plasma samples with coefficient estimations higher than 0.99 with a linearity range of 50-5000 ng mL-1. The relative standard deviation for intra- (n=3) and inter-(n=9) day was 4.2% and 5.7%, respectively. Conclusion: It could be concluded that the application of this method for dose monitoring can be done at hospital and healthcare facilities.
Verapamil, Solvent bar, Microexteraction, Minitab, HPLC-UV
Active pharmaceutical ingredients research center, Pharmaceutical Sciences Research Center, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Active pharmaceutical ingredients research center, Pharmaceutical Sciences Research Center, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran