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A Green Liquid Chromatographic Method For The Simultaneous Determination of Chlorpheniramine Maleate, Pseudoephedrine Hydrochloride and Propyphenazone

Author(s):

Nadia M. Mostafa, Ghada M. Elsayed, Nagiba Hassan and Dina A. El Mously*   Pages 1 - 7 ( 7 )

Abstract:


Background: The concept of green analytical chemistry prevails due to the growing environmental pollution. Objective: Our attempts are to develop simple and eco-friendly method which is non-harmful to the environment by producing minimal waste. In this context, a green liquid chromatographic method was applied for the simultaneous determination of chlorpheniramine maleate, pseudoephedrine hydrochloride and propyphenazone in their combined dosage form. Methods: Separation was carried out using X select HSS RP C18 analytical column (250 × 4.6 mm, 5μm) using methanol - 0.02 M phosphate buffer pH 3 - triethylamine (60:40: 0.1, by volume) as a mobile phase. The separated peaks were detected at 215 nm at a flow rate 1.0 mL/min. Results: Quantification was done over the concentration ranges of 1-25 μg/mL for chlorpheniramine maleate, 5-35 μg/mL for pseudoephedrine hydrochloride and 10-120 μg/mL for propyphenazone. The suggested method was validated with regard to linearity, accuracy and precision according to the International Conference on Harmonization guidelines with good results. Conclusion: It could be used as a safer alternative for routine analysis of the mentioned drugs in quality control laboratories.

Keywords:

Chlorpheniramine maleate, Green chemistry, liquid chromatography, propyphenazone, pseudoephedrine hydrochloride

Affiliation:

Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El Aini, 11562 Cairo, Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El Aini, 11562 Cairo, Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El Aini, 11562 Cairo, Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El Aini, 11562 Cairo



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