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Aptamer-based Homogeneous Analysis for Food Control

Author(s):

Xuhan Xia, Qiang He, Yi Dong, Ruijie Deng and Jinghong Li*   Pages 1 - 9 ( 9 )

Abstract:


Background: Highly sensitive and rapid analysis of food contaminants is of great significance for food safety control. Aptamer is a new kind of recognition molecules which could be applied for constructing homogeneous analysis assays, potentially achieving highly sensitive, cheap and rapid profiling of food contaminants. Methods: An overview of the literature concerning the homogeneous analysis of food contaminations based on aptamers has been reviewed (focused on the most recent literature, 2000-2018). Results: Attributed to aptamer’s controllability, designability and feasibility for the adoption of nucleic acid amplification, rapid, highly sensitive homogeneous assay for various food contaminants could be constructed. The structure-switching aptamer probe would confer quick, efficient and specific response to target food contaminants. Besides, the capability of amplification of aptamer sequences or nucleic acid probes would lead to highly sensitive detection. Conclusions: Aptamer-based homogeneous analysis methods have already been applied to detect various food contaminations ranging from toxins, heavy metal and pesticide to allergen and pathogenic bacteria. However, it is still a challenge to achieve robust and accurate detection of food contaminants in complex food samples.

Keywords:

aptamer, nucleic acid amplification, fluorescence analysis, homogeneous detection, food safety

Affiliation:

College of Light Industry, Textile and Food Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, College of Light Industry, Textile and Food Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, College of Light Industry, Textile and Food Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, College of Light Industry, Textile and Food Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084



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