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Optimization and Analysis of Acid Treated Trimethylamine using Surface Response and Gas Chromatography Analytical Methods

Author(s):

Seul-Ki Park, Fazlurrahman Khan, Yeon-Jin Cho, Dong-Lee Hong, Yu-Mi Jang and Young-Mog Kim*   Pages 1 - 9 ( 9 )

Abstract:


Background: Trimethylamine (TMA) is a nitrogenous base aliphatic organic compound accounting for the odor of rotten fish and used as an indicator for analyzing the quality of fish products.

Introduction: Extraction procedures and analytical methods including colorimetric and gas-chromatography (GC) can quantify the TMA contents of fish products after pre-treatment with basic solutions. However, the extraction procedure and analytical methods for acid-treated samples are not known, despite the majority of fish products being preserved using acid preservatives.

Method: The methodologies used included solid-phase micro-extraction of TMA followed by its quantification by a GC-based analytical method. An analysis of response surface methodology was also conducted to verify the optimum conditions for TMA detection in acid-treated liquid samples affected by factors including trapping time, temperature, and stirring speed.

Results: The results obtained from this study showed that the optimum conditions for the best yield of TMA extraction are 20 min of trapping, emission at 55°C, and stirring at 400 rpm. The validation of the developed method was carried out using rotten fish after acid treatment. Acid treatment decreased TMA by up to 73.01%, however, when adding NaOH solution of the same volume to the samples, TMA increased similar to the control group.

Conclusion: Here, we are report a simple, sensitive, and rapid extraction procedure. A GC-based analytical method was developed for the analysis of TMA from the acid-treated sample. The developed extraction procedure and analytical methods were optimized and validated, which could be helpful for the extraction of TMA without damaging the sample.

Keywords:

Trimethylamine, Solid-phase micro-extraction, Response surface methodology, Box-Behnken design, TMA extraction, Gas chromatography

Affiliation:

Department of Food Science and Technology, Pukyong National University, Busan 48513, Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Department of Food Science and Technology, Pukyong National University, Busan 48513, Department of Food Science and Technology, Pukyong National University, Busan 48513, Department of Food Science and Technology, Pukyong National University, Busan 48513, Department of Food Science and Technology, Pukyong National University, Busan 48513



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