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Automated Post-Column Sample Manipulation Prior to Detection in Liquid Chromatography: A Review of Pharmaceutical and Bioanalytical Applications

Author(s):

Natalia Manousi and Constantinos K. Zacharis *   Pages 1 - 17 ( 17 )

Abstract:


Automated post-column sample manipulation is undoubtedly one of the most challenging approaches in liquid chromatography towards the improvement of method selectivity and sensitivity. With the post-column analyte derivatization being the most-abundant approach of this category other strategies typically comprise post-column infusion of internal standard or other reagents prior to mass spectrometric detection to enhance the ionization efficiency of the analyte or to compensate the ion suppression/enhancement.

In this review, on-line post column methodologies focused on the quality control of pharmaceuticals and biomedical applications will be presented and discussed. Emphasis will be given on the automation capabilities of such systems.

Keywords:

Liquid chromatography, automation, post-column, derivatization, infusion, internal standard, pharmaceuticals, bioanalysis.

Affiliation:

Analytical Development Laboratory, R&D API Operations, Pharmathen SA, 9th klm Thessaloniki-Thermi, Thessaloniki 57001, Analytical Development Laboratory, R&D API Operations, Pharmathen SA, 9th klm Thessaloniki-Thermi, Thessaloniki 57001



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