Laleh Samini, Maryam Khoubnasabjafari, Mohamad M. Alimorad, Vahid Jouyban-Gharamaleki, Hak-Kim Chan and Abolghasem Jouyban* Pages 1032 - 1040 ( 9 )
Background: Analysis of drug concentrations in biological fluids is required in clinical sciences for various purposes. Among other biological samples, exhaled breath condensate (EBC) is a potential sample for follow up of drug concentrations.
Methods: A dispersive liquid-liquid microextraction (DLLME) procedure followed by a validated liquid chromatography method was employed for the determination of budesonide (BDS) in EBC samples collected using a homemade setup. EBC is a non-invasive biological sample with possible applications for monitoring drug concentrations. The proposed analytical method is validated according to the FDA guidelines using EBC-spiked samples. Its applicability is tested on EBC samples collected from healthy volunteers receiving a single puff of BDS.
Results: The best DLLME conditions involved the use of methanol (1 mL) as a disperser solvent, chloroform (200 μL) as an extraction solvent, and centrifugation rate of 3500 rpm for 5 minutes. The method was validated over a concentration range of 21-210 μg·L-1 in EBC. Inter- and intra-day precisions were less than 10% where the acceptable levels are less than 20%. The validated method was successfully applied for the determination of BDS in EBC samples.
Conclusion: The findings of this study indicate that the developed method can be used for the extraction and quantification of BDS in EBC samples using a low cost method.
Bioanalysis, budesonide, determination, exhaled breath condensate, HPLC-UV, microextraction.
Research and Development Department, Jaber Ebne Hayyan Pharmaceutical Co., Tehran, Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Research and Development Department, Jaber Ebne Hayyan Pharmaceutical Co., Tehran, Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, School of Pharmacy, Faculty of Medicine and Health, University of Sydney, Sydney, NSW 2006, Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz