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Imprinted electrochemical sensor of tyrosine based on chitosan/β-cyclodextrin/multi-walled carbon nanotubes composite film

Author(s):

Xiaopeng Bai, Ying Wu, Lili Deng, Li Gong, Tianchi Xu, Wenbo Song and Xun Feng*  

Abstract:


Background: As a vital amino acid in the human body, tyrosine is indispensable in various biological processes, and therefore its accurate and simple determination is of crucial importance. In this work, a facile approach was developed to construct a molecularly imprinted sensor for tyrosine via co-electrodeposition of chitosan, β-cyclodextrin and tyrosine on the surface of indium tin oxide that was pre-coated with multi-walled carbon nanotubes (MWNTs).

Methods: Benefitting from the excellent film-forming ability and the rich functional groups to form a hydrogen bond with target molecules, chitosan was utilized to form a recognition matrix. MWNTs and β-cyclodextrin were then introduced to enhance the selectivity and sensitivity to tyrosine, due to the subtle electronic, catalytic properties and possible π-π interaction of MWNTs with tyrosine, as well as recognition ability of β-cyclodextrin. The morphology of the imprinted films was characterized by a scanning electron microscope. The electrochemistry and tyrosine sensing performance were investigated in detail by cyclic voltammetry and chronoamperometry.

Results: Amperometry results showed that the imprinted sensor exhibited a linear range of 1.0×10−6 to 1.0×10−4 M and 1.0×10−4 to 1.0×10−3 M for tyrosine determination, with a detection limit of 6.0 × 10−7 M (S/N=3). Moreover, a satisfactory recovery in the range of 99.0% to 105.1% was obtained with the application of the imprinted sensor in artificial urine samples analysis.

Conclusion: The imprinted electrode is reusable with satisfactory reproducibility and stability in tyrosine determination.

Keywords:

Molecularly imprinted polymer, tyrosine, chitosan, multi-walled carbon nanotubes, β-cyclodextrin

Affiliation:

Department of Sanitary Inspection, School of Public Health, Shenyang Medical College, Shenyang 110034, College of Chemistry, Jilin University, Changchun 130012, Public Health Experimental Center, Shenyang Medical College, Shenyang 110034, Department of Sanitary Inspection, School of Public Health, Shenyang Medical College, Shenyang 110034, Department of Sanitary Inspection, School of Public Health, Shenyang Medical College, Shenyang 110034, College of Chemistry, Jilin University, Changchun 130012, Department of Sanitary Inspection, School of Public Health, Shenyang Medical College, Shenyang 110034



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