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Dual-opposite Injection Capillary Electrophoresis for the Simultaneous Separation of Anionic and Cationic Compounds

[ Vol. 1 , Issue. 2 ]


Audrius Padarauskas   Pages 149 - 156 ( 8 )


The basic principles, instrumental developments and applications of dual-opposite injection capillary electrophoresis (DOI-CE) technique are reviewed. Two different dual-opposite injection modes are described: electrokinetic injection and sequential hydrodynamic injection. The features of both injection principles are compared, followed by a detailed discussion on the biases of electrokinetic injection and how to reduce them. Flow injection system for automated dual-opposite injection is also briefly discussed. The approaches to the manipulation of the separation selectivity of anionic and cationic compounds in DOI-CE with indirect UV, mixed direct/indirect UV and contactless conductometric detection are highlighted. Finally, the performance of DOI-CE technique is illustrated by a number of practically relevant applications.


capillary electrophoresis, dual-opposite injection, anions, cations, simultaneous separation, review


Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, LT- 03225 Vilnius, Lithuania.

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