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Bottom-Up Proteomics

[ Vol. 5 , Issue. 2 ]

Author(s):

Andrea Armirotti   Pages 116 - 130 ( 15 )

Abstract:


In this work, the “bottom-up” protein identification process is described, from sample digestion to the final database search result. Both MALDI and LC-MS approaches to the identification issue are illustrated. The state-of-the-art of these technique is outlined along with several recent applications, such as MSn data acquisition and chemical derivatization of peptides.

Keywords:

Proteomics, BOTTOM UP PROCESS, MALDI, LC-MS

Affiliation:

Department of Experimental Medicine, Biochemistry Section, University of Genoa, Viale Benedetto XV, 7 Genova, Italy.



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