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Separation of Indole-3-acetic Acid from Tryptophan by Bulk Liquid Membrane

[ Vol. 17 , Issue. 6 ]

Author(s):

Ioana Diaconu, Hassan Y. Aboul-Enein*, Cristina Orbeci, Andrei A. Bunaciu, Ecaterina A. Serban and Elena Ruse   Pages 883 - 891 ( 9 )

Abstract:


Background: Indole-3-acetic acid (IAA) is an important growth hormone for plants obtained by biosynthesis from tryptophan.

Aim: In this paper was studied the competitive transport of two biologically active compounds, indole-3-acetic acid (IAA) and tryptophan (TRP), through a liquid membrane.

Methods: The separation of the two compounds was obtained using a hybrid liquid membrane system having trioctylphosphine oxide (TOPO) as a carrier.

Results: The most important operational parameters of the system, and pH influence on the efficiency of the transport process, in correlation with the speciation diagrams of the two compounds: TRP and IAA, were studied. The evaluation of the transport process was performed by calculating the composition of the phases at the end of the transport process and the organic substrate flow at the membranes exit.

Conclusion: Due to the transport efficiency of over 90% in the case of IAA and the high selectivity at the transport between IAA and TRP, the procedure can be applied for the preparation of a sample containing these analytes.

Keywords:

Bulk liquid membranes, indole-3-acetic acid, tryptophan, membrane separations, biosynthesis, trioctylphosphine oxide.

Affiliation:

University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, 1-7 Polizu Street, Bucharest - 1, 011061, Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Center, Dokki, Cairo 12622, University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, 1-7 Polizu Street, Bucharest - 1, 011061, S.C. AAB_IR Research SRL, 7 Modoran Ene Street, Bucharest - 5, 051832, National Research and Development Institute for Industrial Ecology ECOIND, 71-73 Drumul Podul Dambovitei, Bucharest - 6, 060652, University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, 1-7 Polizu Street, Bucharest - 1, 011061

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